Micro Mornings

B180 BBB


Doors open at 9:00am for socializing and breakfast

Wednesday, January 10, 2018

“GETting tail-anchored proteins to the ER membrane”

Michelle Fry

Clemons Lab

For a cell, making membrane proteins (MPs) is a complicated but critical process. All proteins are made by ribosomes in the cytosol and MPs, accounting for ~30% of the proteins encoded in the eukaryotic genome, must be targeted to their respective sub-cellular membranes. In yeast, a unique class of MPs, tail-anchored (TA) proteins, are targeted to the ER membrane via the Guided Entry of TA proteins (GET) pathway. Loss of function mutations to GET proteins and their mammalian homologs are deleterious and have been linked to a variety of diseases. Although the role of each component has been studied extensively, the structure and binding mechanisms of some remain elusive.

“Study of cellular structures in-situ using a combination of focussed ion beam milling and electron cryotomography”

Shrawan Kumar Mageswaran

Jensen Lab

Electron cryotomography (ECT) is a powerful technique to study cellular ultrastructures in 3D in near native context. However, much of the work has been restricted to small organisms, mainly rod-shaped bacterial cells owing to the sample size limitation imposed by the use of electrons. Cryosectioning followed by ECT has been successfully applied to a few thicker samples but sub-optimal sample preservation limits its overall potential. Here, we show successful application of ECT following a technique named focused ion beam (FIB) milling to study ultrastructures in Saccharomyces cerevisiae. The high-resolution structures (of nuclear pore complexes, spindle pole bodies (SPB) and eisosomes to name a few) revealed by this hybrid technique help us gain new insights about these biological systems and underscores the potential of this technique.

Upcoming MicroMornings

Wednesday, February 14, 2018

Phillips and Tirrell Labs

Wednesday, March 14, 2018

Gradinaru and Ismagilov Labs